BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD279 (PD-1)

Description

Two-color flow cytometric analysis of CD279 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/ 563548 and either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 567397/567398; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color pseudocolor density plots show the correlated expression of CD279 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of CD279 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/ 563548 and either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 567397/567398; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color pseudocolor density plots show the correlated expression of CD279 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of CD279 expression on human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/ 563548 and either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 567397/567398; Right Plot . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The two-color pseudocolor density plots show the correlated expression of CD279 or Ig Isotype control staining versus CD3 for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.