BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD98

Description

Flow cytometric analysis of CD98 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human CD98 antibody Cat. No. 569024/569025; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD98 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD98 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human CD98 antibody Cat. No. 569024/569025; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD98 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD98 expression on Human peripheral blood leucocyte populations. Fresh whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 and then stained with Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plot or Alexa Fluor 488 Mouse Anti-Human CD98 antibody Cat. No. 569024/569025; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD98 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.