BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human LAG-3 (CD223)

Description

Multicolor flow cytometric analysis of LAG-3 CD223 expression on unstimulated Top Figures and stimulated Bottom Figures human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 555329; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Human Recombinant IL-2 Cat. No. 554603; 10 ng/mL . Unstimulated PBMC from the same donor and stimulated PBMC were stained with BD Horizon BV421 Mouse Anti-Human CD8 Cat. No. 562428/562429; Left Figures , BD Horizon BUV737 Mouse Anti-Human CD279 PD-1 Cat. No. 612791/612792; Right Figures , and Alexa Fluor 488 Mouse Anti-Human LAG-3 CD223 Cat. No. 567610/567611 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Multicolor flow cytometric contour plots showing the correlated expression of LAG-3 CD223 versus CD8, or LAG-3 versus CD279 PD-1 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of LAG-3 CD223 expression on unstimulated Top Figures and stimulated Bottom Figures human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 555329; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Human Recombinant IL-2 Cat. No. 554603; 10 ng/mL . Unstimulated PBMC from the same donor and stimulated PBMC were stained with BD Horizon BV421 Mouse Anti-Human CD8 Cat. No. 562428/562429; Left Figures , BD Horizon BUV737 Mouse Anti-Human CD279 PD-1 Cat. No. 612791/612792; Right Figures , and Alexa Fluor 488 Mouse Anti-Human LAG-3 CD223 Cat. No. 567610/567611 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Multicolor flow cytometric contour plots showing the correlated expression of LAG-3 CD223 versus CD8, or LAG-3 versus CD279 PD-1 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of LAG-3 CD223 expression on unstimulated Top Figures and stimulated Bottom Figures human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 555329; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Human Recombinant IL-2 Cat. No. 554603; 10 ng/mL . Unstimulated PBMC from the same donor and stimulated PBMC were stained with BD Horizon BV421 Mouse Anti-Human CD8 Cat. No. 562428/562429; Left Figures , BD Horizon BUV737 Mouse Anti-Human CD279 PD-1 Cat. No. 612791/612792; Right Figures , and Alexa Fluor 488 Mouse Anti-Human LAG-3 CD223 Cat. No. 567610/567611 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Multicolor flow cytometric contour plots showing the correlated expression of LAG-3 CD223 versus CD8, or LAG-3 versus CD279 PD-1 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative unstimulated or stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software.