BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Mouse H-2K[b]/SIINFEKL

Description

Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histograms or Alexa Fluor 488 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570010/570091; solid line histograms at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histograms or Alexa Fluor 488 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570010/570091; solid line histograms at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histograms or Alexa Fluor 488 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570010/570091; solid line histograms at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histograms or Alexa Fluor 488 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570010/570091; solid line histograms at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histograms or Alexa Fluor 488 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570010/570091; solid line histograms at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of H-2K b /SIINFEKL expression on viable C57BL/6 Mouse splenic leukocytes with or without prior SIINFEKL peptide treatment. Mouse splenic leukocytes were either not pulsed Left Plot or were pulsed Right Plot with SIINFEKL peptide for 2 hours and were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histograms or Alexa Fluor 488 Mouse Anti-Mouse H-2K b /SIINFEKL antibody Cat. No. 570010/570091; solid line histograms at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing H-2K b /SIINFEKL expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.