BD PharmingenTM Alexa FluorTM 488 Rat Anti-Human LRRC15

Description

Flow cytometric analysis of LRRC15 expression on Saos-2 cells. Cells from the Human Saos-2 Osteosarcoma, ATCC HTB-85 cell line were stained with either Alexa Fluor 488 Rat IgG1, kappa Isotype Control Cat. No. 557720; dashed line histogram or Alexa Fluor 488 Rat Anti-Human LRRC15 antibody Cat. No. 572052/572066; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing LRRC15 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of LRRC15 expression on Saos-2 cells. Cells from the Human Saos-2 Osteosarcoma, ATCC HTB-85 cell line were stained with either Alexa Fluor 488 Rat IgG1, kappa Isotype Control Cat. No. 557720; dashed line histogram or Alexa Fluor 488 Rat Anti-Human LRRC15 antibody Cat. No. 572052/572066; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing LRRC15 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of LRRC15 expression on Saos-2 cells. Cells from the Human Saos-2 Osteosarcoma, ATCC HTB-85 cell line were stained with either Alexa Fluor 488 Rat IgG1, kappa Isotype Control Cat. No. 557720; dashed line histogram or Alexa Fluor 488 Rat Anti-Human LRRC15 antibody Cat. No. 572052/572066; solid line histogram at 0.5 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing LRRC15 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.