BD PharmingenTM Alexa FluorTM 488 Rat Anti-Mouse CD326

Description

Multiparameter flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676 or Alexa Fluor 488 Rat Anti-Mouse CD326 antibody Cat. No. 567664 at 1.0 mug/test. Left Plot: Thymocytes were further stained with APC Rat Anti-Mouse CD4 553051/561091 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies. The bivariate pseudocolor density plot showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e was derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676 or Alexa Fluor 488 Rat Anti-Mouse CD326 antibody Cat. No. 567664 at 1.0 mug/test. Left Plot: Thymocytes were further stained with APC Rat Anti-Mouse CD4 553051/561091 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies. The bivariate pseudocolor density plot showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e was derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676 or Alexa Fluor 488 Rat Anti-Mouse CD326 antibody Cat. No. 567664 at 1.0 mug/test. Left Plot: Thymocytes were further stained with APC Rat Anti-Mouse CD4 553051/561091 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies. The bivariate pseudocolor density plot showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e was derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676 or Alexa Fluor 488 Rat Anti-Mouse CD326 antibody Cat. No. 567664 at 1.0 mug/test. Left Plot: Thymocytes were further stained with APC Rat Anti-Mouse CD4 553051/561091 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies. The bivariate pseudocolor density plot showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e was derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676 or Alexa Fluor 488 Rat Anti-Mouse CD326 antibody Cat. No. 567664 at 1.0 mug/test. Left Plot: Thymocytes were further stained with APC Rat Anti-Mouse CD4 553051/561091 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies. The bivariate pseudocolor density plot showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e was derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD326 expression on mouse thymocytes and splenic T lymphocytes. BALB/c mouse thymocytes and splenic leucocytes were separately preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676 or Alexa Fluor 488 Rat Anti-Mouse CD326 antibody Cat. No. 567664 at 1.0 mug/test. Left Plot: Thymocytes were further stained with APC Rat Anti-Mouse CD4 553051/561091 and BD Horizon BUV395 Rat Anti-Mouse CD8a Cat. No. 563786 antibodies. The CD326 solid line histogram and Ig Isotype Control dashed line histogram fluorescence histograms were derived from CD4- and CD8-negative gated events with the forward and side light-scatter characteristics of viable thymocytes. Middle and Right Plots: The splenic leucocytes were further stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e Cat. No. 563565/565992 and APC Rat Anti-Mouse CD25 Cat. No. 557192 antibodies. The bivariate pseudocolor density plot showing the correlated expression of Ig Isotype control staining Middle Plot or CD326 Right Plot versus CD3e was derived from CD25+ gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. A small population of CD3+CD25+CD326+ cells was detected Right Plot , whereas the CD25- T cells do not express detectable levels of CD326 data not shown . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.