BD PharmingenTM Alexa FluorTM 488 Rat Anti-Mouse CD34

Description

Multiparameter analysis of of CD34+ and CD34- subpopulations of mouse bone marrow hematopoietic progenitor cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Mouse CD34 antibody Cat. No. 567135; Right Plot at 2 mug/test followed by staining with a cocktail of BD Horizon BV421-conjugated antibodies specific for CD3e Cat. No. 562600 , CD11b 562605 , CD45R/B220 562922 , TER-119/Erythroid Cells 563998 , and Ly-6G and Ly-6C 562709 , markers that identified major lineage-committed cell types. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus the Lineage Cocktail of Markers was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter analysis of of CD34+ and CD34- subpopulations of mouse bone marrow hematopoietic progenitor cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Mouse CD34 antibody Cat. No. 567135; Right Plot at 2 mug/test followed by staining with a cocktail of BD Horizon BV421-conjugated antibodies specific for CD3e Cat. No. 562600 , CD11b 562605 , CD45R/B220 562922 , TER-119/Erythroid Cells 563998 , and Ly-6G and Ly-6C 562709 , markers that identified major lineage-committed cell types. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus the Lineage Cocktail of Markers was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter analysis of of CD34+ and CD34- subpopulations of mouse bone marrow hematopoietic progenitor cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Mouse CD34 antibody Cat. No. 567135; Right Plot at 2 mug/test followed by staining with a cocktail of BD Horizon BV421-conjugated antibodies specific for CD3e Cat. No. 562600 , CD11b 562605 , CD45R/B220 562922 , TER-119/Erythroid Cells 563998 , and Ly-6G and Ly-6C 562709 , markers that identified major lineage-committed cell types. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus the Lineage Cocktail of Markers was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter analysis of of CD34+ and CD34- subpopulations of mouse bone marrow hematopoietic progenitor cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Mouse CD34 antibody Cat. No. 567135; Right Plot at 2 mug/test followed by staining with a cocktail of BD Horizon BV421-conjugated antibodies specific for CD3e Cat. No. 562600 , CD11b 562605 , CD45R/B220 562922 , TER-119/Erythroid Cells 563998 , and Ly-6G and Ly-6C 562709 , markers that identified major lineage-committed cell types. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus the Lineage Cocktail of Markers was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter analysis of of CD34+ and CD34- subpopulations of mouse bone marrow hematopoietic progenitor cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Mouse CD34 antibody Cat. No. 567135; Right Plot at 2 mug/test followed by staining with a cocktail of BD Horizon BV421-conjugated antibodies specific for CD3e Cat. No. 562600 , CD11b 562605 , CD45R/B220 562922 , TER-119/Erythroid Cells 563998 , and Ly-6G and Ly-6C 562709 , markers that identified major lineage-committed cell types. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus the Lineage Cocktail of Markers was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter analysis of of CD34+ and CD34- subpopulations of mouse bone marrow hematopoietic progenitor cells. BALB/c mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either Alexa Fluor 488 Rat IgG2a, kappa Isotype Control Cat. No. 557676; Left Plot or Alexa Fluor 488 Mouse CD34 antibody Cat. No. 567135; Right Plot at 2 mug/test followed by staining with a cocktail of BD Horizon BV421-conjugated antibodies specific for CD3e Cat. No. 562600 , CD11b 562605 , CD45R/B220 562922 , TER-119/Erythroid Cells 563998 , and Ly-6G and Ly-6C 562709 , markers that identified major lineage-committed cell types. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus the Lineage Cocktail of Markers was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.