BD PharmingenTM Alexa FluorTM 647 Biosimilar Anti-Human CD20 Rituximab297

Description

Two-color flow cytometric analysis of Human CD20 expression on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody Cat. No. 555413 and with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569961; Left Plot or Alexa Fluor 647 Human Anti-Human CD20 Rituximab N297A Biosimilar antibody Cat. No. 569959/569960; Right Plot . After staining, the cells were treated with 1 BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Human CD20 expression on Human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569961; Left Plot or Alexa Fluor 647 Human Anti-Human CD20 Rituximab N297A Biosimilar antibody Cat. No. 569959/569960; Right Plot . After staining, the cells were treated with 1 BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of Human CD20 expression on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody Cat. No. 555413 and with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569961; Left Plot or Alexa Fluor 647 Human Anti-Human CD20 Rituximab N297A Biosimilar antibody Cat. No. 569959/569960; Right Plot . After staining, the cells were treated with 1 BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of Human CD20 expression on Human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569961; Left Plot or Alexa Fluor 647 Human Anti-Human CD20 Rituximab N297A Biosimilar antibody Cat. No. 569959/569960; Right Plot . After staining, the cells were treated with 1 BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of Human CD20 expression on Human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD19 antibody Cat. No. 555413 and with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569961; Left Plot or Alexa Fluor 647 Human Anti-Human CD20 Rituximab N297A Biosimilar antibody Cat. No. 569959/569960; Right Plot . After staining, the cells were treated with 1 BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of Human CD20 expression on Human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569961; Left Plot or Alexa Fluor 647 Human Anti-Human CD20 Rituximab N297A Biosimilar antibody Cat. No. 569959/569960; Right Plot . After staining, the cells were treated with 1 BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The bivariate pseudocolor density plot showing the correlated expression of CD20 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.