BD PharmingenTM Alexa FluorTM 647 Biosimilar Anti-Human TNF

Description

Multiparameter flow cytometric analysis of TNF expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569794; Left Plot or Alexa Fluor 647 Biosimilar Anti-Human TNF antibody Infliximab N297A Biosimilar.rMAb Cat. No. 570532/570533; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Multiparameter flow cytometric analysis of TNF expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569794; Left Plot or Alexa Fluor 647 Biosimilar Anti-Human TNF antibody Infliximab N297A Biosimilar.rMAb Cat. No. 570532/570533; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Multiparameter flow cytometric analysis of TNF expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569794; Left Plot or Alexa Fluor 647 Biosimilar Anti-Human TNF antibody Infliximab N297A Biosimilar.rMAb Cat. No. 570532/570533; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Show More Multiparameter flow cytometric analysis of TNF expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569794; Left Plot or Alexa Fluor 647 Biosimilar Anti-Human TNF antibody Infliximab N297A Biosimilar.rMAb Cat. No. 570532/570533; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Show More Multiparameter flow cytometric analysis of TNF expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569794; Left Plot or Alexa Fluor 647 Biosimilar Anti-Human TNF antibody Infliximab N297A Biosimilar.rMAb Cat. No. 570532/570533; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software. Multiparameter flow cytometric analysis of TNF expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Human IgG1 N297A , kappa Isotype Control Cat. No. 569794; Left Plot or Alexa Fluor 647 Biosimilar Anti-Human TNF antibody Infliximab N297A Biosimilar.rMAb Cat. No. 570532/570533; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD Fortessa X-20 Flow Cytometer System and FlowJo software.