BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Cyclin A

Description

Flow cytometric analysis of Cyclin A expression in Human MOLT-4 Top Plots and Mouse NIH-3T3 cells Bottom Plots . Cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plots or Alexa Fluor 647 Mouse Anti-Cyclin A antibody Cat. No. 568280/568281; Right Plots at 1 mug/test. The bivariate pseudocolor density plots showing the correlated expression of Cyclin A or Ig Isotype control versus DNA DAPI were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Cyclin A expression in Human MOLT-4 Top Plots and Mouse NIH-3T3 cells Bottom Plots . Cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plots or Alexa Fluor 647 Mouse Anti-Cyclin A antibody Cat. No. 568280/568281; Right Plots at 1 mug/test. The bivariate pseudocolor density plots showing the correlated expression of Cyclin A or Ig Isotype control versus DNA DAPI were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Cyclin A expression in Human MOLT-4 Top Plots and Mouse NIH-3T3 cells Bottom Plots . Cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line and Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were fixed and permeabilized using BD Cytofix Fixation Buffer Cat. No. 554655 and BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then stained with DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 to stain DNA and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plots or Alexa Fluor 647 Mouse Anti-Cyclin A antibody Cat. No. 568280/568281; Right Plots at 1 mug/test. The bivariate pseudocolor density plots showing the correlated expression of Cyclin A or Ig Isotype control versus DNA DAPI were derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.