BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human CD105 (Endoglin)

Description

Multiparameter flow cytometric analysis of CD105 Endoglin expression on Human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 568987/568988; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD105 Endoglin or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD105 Endoglin expression on Human U937 cells. Cells from the human U937 Histiocytic lymphoma, ATCC CRL-1593 cell line were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 568987/568988; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD105 Endoglin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of 7-AAD-negative viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD105 Endoglin expression on Human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 568987/568988; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD105 Endoglin or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD105 Endoglin expression on Human U937 cells. Cells from the human U937 Histiocytic lymphoma, ATCC CRL-1593 cell line were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 568987/568988; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD105 Endoglin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of 7-AAD-negative viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD105 Endoglin expression on Human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 568987/568988; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of CD105 Endoglin or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD105 Endoglin expression on Human U937 cells. Cells from the human U937 Histiocytic lymphoma, ATCC CRL-1593 cell line were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human CD105 Endoglin antibody Cat. No. 568987/568988; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD105 Endoglin expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of 7-AAD-negative viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.