BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human CD138

Description

Multiparameter flow cytometric analysis of CD138 expression on viable Human U266 myeloma cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564220 and stained with either Alexa Fluor A647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dotted line histogram or Alexa Fluor A647 Mouse Anti-CD138 antibody Cat. No. 570743/570744; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD138 expression or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative U266 cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD138 expression on viable Human lymphocytes. Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564220 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD38 Cat. No. 562444 and Alexa Fluor A647 Mouse Anti-Human CD138 Cat. No. 564219/564220 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD138 versus CD38 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD138 expression on viable Human U266 myeloma cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564220 and stained with either Alexa Fluor A647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dotted line histogram or Alexa Fluor A647 Mouse Anti-CD138 antibody Cat. No. 570743/570744; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD138 expression or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative U266 cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD138 expression on viable Human lymphocytes. Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564220 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD38 Cat. No. 562444 and Alexa Fluor A647 Mouse Anti-Human CD138 Cat. No. 564219/564220 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD138 versus CD38 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD138 expression on viable Human U266 myeloma cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564220 and stained with either Alexa Fluor A647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dotted line histogram or Alexa Fluor A647 Mouse Anti-CD138 antibody Cat. No. 570743/570744; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD138 expression or Ig Isotype control staining was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative U266 cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD138 expression on viable Human lymphocytes. Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block Cat. No. 564220 . The cells were then stained with BD Horizon BV421 Mouse Anti-Human CD38 Cat. No. 562444 and Alexa Fluor A647 Mouse Anti-Human CD138 Cat. No. 564219/564220 antibodies. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD138 versus CD38 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software.