BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human CD34

Description

Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were preincubated in the presence of Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with FITC Mouse Anti-Human CD14 Cat. No. 555397/557153 , PE Mouse Anti-Human CD133 Cat. No. 566593/566594 antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD34 antibody Cat No. 568771/568772; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus CD133 was derived from CD14-negative gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were preincubated in the presence of Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with FITC Mouse Anti-Human CD14 Cat. No. 555397/557153 , PE Mouse Anti-Human CD133 Cat. No. 566593/566594 antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD34 antibody Cat No. 568771/568772; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus CD133 was derived from CD14-negative gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multicolor flow cytometric analysis of CD34 expression on Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were preincubated in the presence of Human BD Fc Block Cat. No. 564219/564220 . The cells were then stained with FITC Mouse Anti-Human CD14 Cat. No. 555397/557153 , PE Mouse Anti-Human CD133 Cat. No. 566593/566594 antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD34 antibody Cat No. 568771/568772; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD34 or Ig Isotype control staining versus CD133 was derived from CD14-negative gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.