BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human CD47

Description

Multiparameter flow cytometric analysis of CD47 expression on human peripheral blood leucocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor647 Mouse Anti-Human CD47 antibody Cat. No. 568340/568341; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD47 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD47 expression on human peripheral blood leucocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor647 Mouse Anti-Human CD47 antibody Cat. No. 568340/568341; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD47 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD47 expression on human peripheral blood leucocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor647 Mouse Anti-Human CD47 antibody Cat. No. 568340/568341; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD47 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD47 expression on human peripheral blood leucocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor647 Mouse Anti-Human CD47 antibody Cat. No. 568340/568341; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD47 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD47 expression on human peripheral blood leucocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor647 Mouse Anti-Human CD47 antibody Cat. No. 568340/568341; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD47 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD47 expression on human peripheral blood leucocyte populations. Human peripheral blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. After washing, the leucocytes were stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor647 Mouse Anti-Human CD47 antibody Cat. No. 568340/568341; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD47 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.