BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human CD59

Description

Multiparameter Flow Cytometric Analysis of CD59 Expression on Human Leucocytes Left Panel: CD59 expression on different leucocyte populations. Whole blood was stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; Top Plot or Alexa Fluor 647 Mouse Anti-Human CD59 antibody Cat. No. 568270; Bottom Plot Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The pseudocolor density plot showing the correlated expression of CD59 or Ig isotype control versus side light-scatter signals SSC was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Panel: CD59 expression on different lymphocyte subpopulations. Whole blood was similarly stained with BD Horizon BUV395 Mouse Anti-Human CD3 Cat. No. 563546/563548 and PE Mouse Anti-Human NCAM-1 CD56 Cat. No. 563238 antibodies and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Top Plots or Alexa Fluor 647 Mouse Anti-Human CD59 antibody Bottom Plots . Erythrocytes were similarly lysed. The pseudocolor density plots showing the correlated expression of CD59 or Ig isotype control versus CD3 Left Plots or versus CD56 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter Flow Cytometric Analysis of CD59 Expression on Human Leucocytes Left Panel: CD59 expression on different leucocyte populations. Whole blood was stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; Top Plot or Alexa Fluor 647 Mouse Anti-Human CD59 antibody Cat. No. 568270; Bottom Plot Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The pseudocolor density plot showing the correlated expression of CD59 or Ig isotype control versus side light-scatter signals SSC was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Panel: CD59 expression on different lymphocyte subpopulations. Whole blood was similarly stained with BD Horizon BUV395 Mouse Anti-Human CD3 Cat. No. 563546/563548 and PE Mouse Anti-Human NCAM-1 CD56 Cat. No. 563238 antibodies and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Top Plots or Alexa Fluor 647 Mouse Anti-Human CD59 antibody Bottom Plots . Erythrocytes were similarly lysed. The pseudocolor density plots showing the correlated expression of CD59 or Ig isotype control versus CD3 Left Plots or versus CD56 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter Flow Cytometric Analysis of CD59 Expression on Human Leucocytes Left Panel: CD59 expression on different leucocyte populations. Whole blood was stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; Top Plot or Alexa Fluor 647 Mouse Anti-Human CD59 antibody Cat. No. 568270; Bottom Plot Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The pseudocolor density plot showing the correlated expression of CD59 or Ig isotype control versus side light-scatter signals SSC was derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Right Panel: CD59 expression on different lymphocyte subpopulations. Whole blood was similarly stained with BD Horizon BUV395 Mouse Anti-Human CD3 Cat. No. 563546/563548 and PE Mouse Anti-Human NCAM-1 CD56 Cat. No. 563238 antibodies and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Top Plots or Alexa Fluor 647 Mouse Anti-Human CD59 antibody Bottom Plots . Erythrocytes were similarly lysed. The pseudocolor density plots showing the correlated expression of CD59 or Ig isotype control versus CD3 Left Plots or versus CD56 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software.