BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human FoxA2

Description

Flow cytometric analysis of FoxA2 expression in Hep G2 cells. Cells from the Human Hep G2 HEPG2 Hepatocellular carcinoma, ATCC HB-8065 cell line were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human FoxA2 antibody Cat. No. 570353/570423; solid line histogram . The fluorescence histogram showing FoxA2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Flow cytometric analysis of FoxA2 expression in Hep G2 cells. Cells from the Human Hep G2 HEPG2 Hepatocellular carcinoma, ATCC HB-8065 cell line were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human FoxA2 antibody Cat. No. 570353/570423; solid line histogram . The fluorescence histogram showing FoxA2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis of FoxA2 expression in Hep G2 cells. Cells from the Human Hep G2 HEPG2 Hepatocellular carcinoma, ATCC HB-8065 cell line were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human FoxA2 antibody Cat. No. 570353/570423; solid line histogram . The fluorescence histogram showing FoxA2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Flow Cytometer System and FlowJo Software.