BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human Lysozyme

Description

Flow cytometric analysis of Lysozyme expression in peripheral blood leucocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Lysozyme antibody Cat. No. 568671; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Lysozyme or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Lysozyme expression in peripheral blood leucocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Lysozyme antibody Cat. No. 568671; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Lysozyme or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Lysozyme expression in peripheral blood leucocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Lysozyme antibody Cat. No. 568671; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Lysozyme or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Lysozyme expression in peripheral blood leucocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Lysozyme antibody Cat. No. 568671; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Lysozyme or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of Lysozyme expression in peripheral blood leucocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Lysozyme antibody Cat. No. 568671; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Lysozyme or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of Lysozyme expression in peripheral blood leucocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and to fix leucocytes. The fixed leucocytes were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Lysozyme antibody Cat. No. 568671; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Lysozyme or Ig Isotype control staining versus side light-scatter signals SSC-A was derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a LSRFortessa X-20 Cell Analyzer System and FlowJo software.