BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human PD-L1 (CD274)

Description

Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568924/568925; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated DAPI-negative viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568924/568925; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated DAPI-negative viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of PD-L1 CD274 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histogram or Alexa Fluor 647 Mouse Anti-Human PD-L1 CD274 antibody Cat. No. 568924/568925; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing the expression of PD-L1 CD274 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated DAPI-negative viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.