BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Human TIGIT

Description

Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 Cat. No. 555516/561903; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and BD Horizon BV421 Mouse Anti-Human CD45RO Cat. No. 562641/562649; Right Plots antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Top Plots or Alexa Fluor 647 Mouse Anti-Human TIGIT antibody Cat. No. 568944/568945; Bottom Plots as indicated. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the light scatter characteristics of intact lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of intact lymphocytes. Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 Cat. No. 555516/561903; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and BD Horizon BV421 Mouse Anti-Human CD45RO Cat. No. 562641/562649; Right Plots antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Top Plots or Alexa Fluor 647 Mouse Anti-Human TIGIT antibody Cat. No. 568944/568945; Bottom Plots as indicated. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the light scatter characteristics of intact lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of intact lymphocytes. Show More Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 NCAM-1 Cat. No. 555516/561903; Middle Plots , BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , and BD Horizon BV421 Mouse Anti-Human CD45RO Cat. No. 562641/562649; Right Plots antibodies, and with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Top Plots or Alexa Fluor 647 Mouse Anti-Human TIGIT antibody Cat. No. 568944/568945; Bottom Plots as indicated. Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the light scatter characteristics of intact lymphocytes. Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT or Ig Isotype control staining versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of intact lymphocytes.