BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Mouse C1q

Description

Flow cytometric analysis of Mouse C1q deposition on BALB/c thymocytes labelled with CD90.2 Thy-1.2 -specific antibody followed by incubation with Mouse serum. BALB/c Mouse thymocytes were labeled with either Purified Rat IgG2b, kappa Isotype Control Cat. No. 555846, Left Plot or Purified Rat Anti-Mouse CD90.2 antibody Cat. No. 553009, Right Plot for 20 min at 4C. The cells were then incubated with Mouse serum as a source of complement in the presence of 5 mM of EDTA, for 30 min at 4C. After washing, the cells were stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; dashed line histograms or Alexa Fluor 647 Mouse Anti-Mouse C1q antibody Cat. No. 571192/571193; solid line histograms at 0.5 mug per test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to the cells right before analysis. The fluorescence histograms showing the deposited levels of Mouse C1q were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C1q deposition on BALB/c thymocytes labelled with CD90.2 Thy-1.2 -specific antibody followed by incubation with Mouse serum. BALB/c Mouse thymocytes were labeled with either Purified Rat IgG2b, kappa Isotype Control Cat. No. 555846, Left Plot or Purified Rat Anti-Mouse CD90.2 antibody Cat. No. 553009, Right Plot for 20 min at 4C. The cells were then incubated with Mouse serum as a source of complement in the presence of 5 mM of EDTA, for 30 min at 4C. After washing, the cells were stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; dashed line histograms or Alexa Fluor 647 Mouse Anti-Mouse C1q antibody Cat. No. 571192/571193; solid line histograms at 0.5 mug per test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to the cells right before analysis. The fluorescence histograms showing the deposited levels of Mouse C1q were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C1q deposition on BALB/c thymocytes labelled with CD90.2 Thy-1.2 -specific antibody followed by incubation with Mouse serum. BALB/c Mouse thymocytes were labeled with either Purified Rat IgG2b, kappa Isotype Control Cat. No. 555846, Left Plot or Purified Rat Anti-Mouse CD90.2 antibody Cat. No. 553009, Right Plot for 20 min at 4C. The cells were then incubated with Mouse serum as a source of complement in the presence of 5 mM of EDTA, for 30 min at 4C. After washing, the cells were stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; dashed line histograms or Alexa Fluor 647 Mouse Anti-Mouse C1q antibody Cat. No. 571192/571193; solid line histograms at 0.5 mug per test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to the cells right before analysis. The fluorescence histograms showing the deposited levels of Mouse C1q were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Mouse C1q deposition on BALB/c thymocytes labelled with CD90.2 Thy-1.2 -specific antibody followed by incubation with Mouse serum. BALB/c Mouse thymocytes were labeled with either Purified Rat IgG2b, kappa Isotype Control Cat. No. 555846, Left Plot or Purified Rat Anti-Mouse CD90.2 antibody Cat. No. 553009, Right Plot for 20 min at 4C. The cells were then incubated with Mouse serum as a source of complement in the presence of 5 mM of EDTA, for 30 min at 4C. After washing, the cells were stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; dashed line histograms or Alexa Fluor 647 Mouse Anti-Mouse C1q antibody Cat. No. 571192/571193; solid line histograms at 0.5 mug per test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to the cells right before analysis. The fluorescence histograms showing the deposited levels of Mouse C1q were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Mouse C1q deposition on BALB/c thymocytes labelled with CD90.2 Thy-1.2 -specific antibody followed by incubation with Mouse serum. BALB/c Mouse thymocytes were labeled with either Purified Rat IgG2b, kappa Isotype Control Cat. No. 555846, Left Plot or Purified Rat Anti-Mouse CD90.2 antibody Cat. No. 553009, Right Plot for 20 min at 4C. The cells were then incubated with Mouse serum as a source of complement in the presence of 5 mM of EDTA, for 30 min at 4C. After washing, the cells were stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; dashed line histograms or Alexa Fluor 647 Mouse Anti-Mouse C1q antibody Cat. No. 571192/571193; solid line histograms at 0.5 mug per test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to the cells right before analysis. The fluorescence histograms showing the deposited levels of Mouse C1q were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Mouse C1q deposition on BALB/c thymocytes labelled with CD90.2 Thy-1.2 -specific antibody followed by incubation with Mouse serum. BALB/c Mouse thymocytes were labeled with either Purified Rat IgG2b, kappa Isotype Control Cat. No. 555846, Left Plot or Purified Rat Anti-Mouse CD90.2 antibody Cat. No. 553009, Right Plot for 20 min at 4C. The cells were then incubated with Mouse serum as a source of complement in the presence of 5 mM of EDTA, for 30 min at 4C. After washing, the cells were stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; dashed line histograms or Alexa Fluor 647 Mouse Anti-Mouse C1q antibody Cat. No. 571192/571193; solid line histograms at 0.5 mug per test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to the cells right before analysis. The fluorescence histograms showing the deposited levels of Mouse C1q were derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.