BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Mouse EVA1

Description

Flow cytometric analysis of EVA1 expression on mouse thymocytes. C57BL/6 mouse thymocytes were stained with PE Anti-Mouse CD4 Cat. No. 553048/553049/561837 , BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BUV395 Rat Anti-Mouse CD25 Cat. No. 564022 , and Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571 or Alexa Fluor 647 Mouse Anti-Mouse EVA1 antibody Cat. No. 567660 at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Isotype control dotted line histogram or EVA1 solid line histogram staining on DN3 cells CD4- CD8a- CD44- CD25+ are shown and compared to EVA1 shaded histogram staining on DN1 cells CD4- CD8a- CD44+ CD25- . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative thymocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of EVA1 expression on mouse thymocytes. C57BL/6 mouse thymocytes were stained with PE Anti-Mouse CD4 Cat. No. 553048/553049/561837 , BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BUV395 Rat Anti-Mouse CD25 Cat. No. 564022 , and Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571 or Alexa Fluor 647 Mouse Anti-Mouse EVA1 antibody Cat. No. 567660 at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Isotype control dotted line histogram or EVA1 solid line histogram staining on DN3 cells CD4- CD8a- CD44- CD25+ are shown and compared to EVA1 shaded histogram staining on DN1 cells CD4- CD8a- CD44+ CD25- . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative thymocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of EVA1 expression on mouse thymocytes. C57BL/6 mouse thymocytes were stained with PE Anti-Mouse CD4 Cat. No. 553048/553049/561837 , BD Horizon BUV737 Rat Anti-Mouse CD8a Cat. No. 612759 , FITC Rat Anti-Mouse CD44 Cat. No. 553133/561859 , BD Horizon BUV395 Rat Anti-Mouse CD25 Cat. No. 564022 , and Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571 or Alexa Fluor 647 Mouse Anti-Mouse EVA1 antibody Cat. No. 567660 at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. Isotype control dotted line histogram or EVA1 solid line histogram staining on DN3 cells CD4- CD8a- CD44- CD25+ are shown and compared to EVA1 shaded histogram staining on DN1 cells CD4- CD8a- CD44+ CD25- . The fluorescence histograms showing the expression of EVA1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative thymocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.