BD PharmingenTM Alexa FluorTM 647 Mouse Anti-MR1

Description

Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either cultured 16 h with Acetyl-6-Formylpterin + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303 or without Ac-6-FP No Ac-6-FP as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 568669/568670; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on B16-F0 cells. Cells from the Mouse B16-F0 Skin Melanoma, ATCC CRL-6322 cell line were either cultured 16 h with Acetyl-6-Formylpterin + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303 or without Ac-6-FP No Ac-6-FP as indicated. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dotted line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 568669/568670; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either cultured 16 h with Acetyl-6-Formylpterin + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303 or without Ac-6-FP No Ac-6-FP as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 568669/568670; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of MR1 expression on B16-F0 cells. Cells from the Mouse B16-F0 Skin Melanoma, ATCC CRL-6322 cell line were either cultured 16 h with Acetyl-6-Formylpterin + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303 or without Ac-6-FP No Ac-6-FP as indicated. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dotted line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 568669/568670; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either cultured 16 h with Acetyl-6-Formylpterin + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303 or without Ac-6-FP No Ac-6-FP as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 568669/568670; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on B16-F0 cells. Cells from the Mouse B16-F0 Skin Melanoma, ATCC CRL-6322 cell line were either cultured 16 h with Acetyl-6-Formylpterin + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303 or without Ac-6-FP No Ac-6-FP as indicated. The cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; dotted line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 568669/568670; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.