BD PharmingenTM Alexa FluorTM 647 Mouse Anti-MR1

Description

Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 570026/570107; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 570026/570107; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 570026/570107; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 570026/570107; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 570026/570107; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of MR1 expression on Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMCs were either not cultured with Acetyl-6-Formylpterin No Ac-6-FP; Left Plot or cultured 16 h with Ac-6-FP + Ac-6-FP; 2.3 mug/ml 10 muM ; Cayman Chemicals Cat. No. 23303; Right Plot as indicated. The cells were then preincubated with Human BD Fc Block Cat. No. 564219/564220 and stained with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565357; dashed line histograms or Alexa Fluor 647 Mouse Anti-MR1 antibody Cat. No. 570026/570107; solid line histograms at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histograms showing MR1 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.