BD PharmingenTM Alexa FluorTM 647 Mouse Anti-Rat CD38

Description

Flow cytometric analysis of CD38 expression on Rat splenic leukocytes. Lewis Rat splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550271 . The cells were then stained with FITC Mouse Anti-Rat CD3 antibody Cat No. 557354 and with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Rat CD38 antibody Cat. No. 569804/569878; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD38 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD38 expression on Rat splenic leukocytes. Lewis Rat splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550271 . The cells were then stained with FITC Mouse Anti-Rat CD3 antibody Cat No. 557354 and with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Rat CD38 antibody Cat. No. 569804/569878; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD38 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD38 expression on Rat splenic leukocytes. Lewis Rat splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550271 . The cells were then stained with FITC Mouse Anti-Rat CD3 antibody Cat No. 557354 and with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Rat CD38 antibody Cat. No. 569804/569878; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD38 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD38 expression on Rat splenic leukocytes. Lewis Rat splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550271 . The cells were then stained with FITC Mouse Anti-Rat CD3 antibody Cat No. 557354 and with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Rat CD38 antibody Cat. No. 569804/569878; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD38 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD38 expression on Rat splenic leukocytes. Lewis Rat splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550271 . The cells were then stained with FITC Mouse Anti-Rat CD3 antibody Cat No. 557354 and with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Rat CD38 antibody Cat. No. 569804/569878; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD38 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD38 expression on Rat splenic leukocytes. Lewis Rat splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550271 . The cells were then stained with FITC Mouse Anti-Rat CD3 antibody Cat No. 557354 and with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Rat CD38 antibody Cat. No. 569804/569878; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD38 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.