BD PharmingenTM Alexa FluorTM 647 Mouse Anti-TRIM

Description

Flow cytometric analysis of TRIM expression on Human Peripheral Blood Mononuclear Cells PBMC . Human PBMC were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-TRIM antibody Cat. No. 568558; Right Plot at 5 mul/test. The bivariate pseudocolor density plot showing the correlated expression of TRIM versus CD3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of TRIM expression on Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063 and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-TRIM antibody Cat. No. 568558 using an optimal dose of 1.25 mul/test. The bivariate pseudocolor density plot showing the correlated expression of TRIM versus CD3e or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of TRIM expression on Human Peripheral Blood Mononuclear Cells PBMC . Human PBMC were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-TRIM antibody Cat. No. 568558; Right Plot at 5 mul/test. The bivariate pseudocolor density plot showing the correlated expression of TRIM versus CD3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of TRIM expression on Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063 and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-TRIM antibody Cat. No. 568558 using an optimal dose of 1.25 mul/test. The bivariate pseudocolor density plot showing the correlated expression of TRIM versus CD3e or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of TRIM expression on Human Peripheral Blood Mononuclear Cells PBMC . Human PBMC were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-TRIM antibody Cat. No. 568558; Right Plot at 5 mul/test. The bivariate pseudocolor density plot showing the correlated expression of TRIM versus CD3 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Flow cytometric analysis of TRIM expression on Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063 and with either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-TRIM antibody Cat. No. 568558 using an optimal dose of 1.25 mul/test. The bivariate pseudocolor density plot showing the correlated expression of TRIM versus CD3e or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.