BD PharmingenTM Alexa FluorTM 647 Rabbit Anti-Human ACAC

Description

Flow cytometric analysis of ACAC expression in SH-SY5Y cells. Cells from the Human SH-SY5Y Neuroblastoma, ATCC CRL-2266 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Rabbit IgG Isotype Control Cat. No. 569345; dashed line histogram or Alexa Fluor 647 Rabbit Anti-Human ACAC antibody Cat. No. 569402; solid line histogram at 0.016 mug/test. The fluorescence histogram showing the expression of ACAC or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of ACAC expression in SH-SY5Y cells. Cells from the Human SH-SY5Y Neuroblastoma, ATCC CRL-2266 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Rabbit IgG Isotype Control Cat. No. 569345; dashed line histogram or Alexa Fluor 647 Rabbit Anti-Human ACAC antibody Cat. No. 569402; solid line histogram at 0.016 mug/test. The fluorescence histogram showing the expression of ACAC or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of ACAC expression in SH-SY5Y cells. Cells from the Human SH-SY5Y Neuroblastoma, ATCC CRL-2266 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Rabbit IgG Isotype Control Cat. No. 569345; dashed line histogram or Alexa Fluor 647 Rabbit Anti-Human ACAC antibody Cat. No. 569402; solid line histogram at 0.016 mug/test. The fluorescence histogram showing the expression of ACAC or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.