BD PharmingenTM Alexa FluorTM 647 Rabbit Anti-Human GLUT1

Description

Flow cytometric analysis of GLUT1 expression in A549 cells. Cells from the Human A549 Lung adenocarcinoma, ATCC CCL-185 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Rabbit IgG Isotype Control Cat. No. 569345; dashed line histogram or Alexa Fluor 647 Rabbit Anti-Human GLUT1 antibody Cat. No. 569293; solid line histogram at 0.06 mug/test. The fluorescence histogram showing the expression of GLUT1 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of GLUT1 expression in A549 cells. Cells from the Human A549 Lung adenocarcinoma, ATCC CCL-185 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Rabbit IgG Isotype Control Cat. No. 569345; dashed line histogram or Alexa Fluor 647 Rabbit Anti-Human GLUT1 antibody Cat. No. 569293; solid line histogram at 0.06 mug/test. The fluorescence histogram showing the expression of GLUT1 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of GLUT1 expression in A549 cells. Cells from the Human A549 Lung adenocarcinoma, ATCC CCL-185 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either Alexa Fluor 647 Rabbit IgG Isotype Control Cat. No. 569345; dashed line histogram or Alexa Fluor 647 Rabbit Anti-Human GLUT1 antibody Cat. No. 569293; solid line histogram at 0.06 mug/test. The fluorescence histogram showing the expression of GLUT1 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.