BD PharmingenTM Alexa FluorTM 647 Rat Anti-Mouse CD14

Description

Flow cytometric analysis of CD14 expression on viable J774A.1 cells. Cells from the J774A Mouse macrophage, ATCC TIB-67 cell line were stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dashed line histogram or Alexa Fluor 647 Rat Anti-Mouse CD14 antibody Cat. No. 570368/570438; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD14 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD14 expression on viable Mouse thioglycolate-elicited peritoneal exudate cell populations. C57BL/6 Mouse peritoneal exudate cells were isolated 3 days post-stimulation by intraperitoneal injection of thioglycolate solution Thio-PEC and were preincubated with BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 .The cells were then stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD14 antibody Cat. No. 570368/570438; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD14 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD14 expression on viable J774A.1 cells. Cells from the J774A Mouse macrophage, ATCC TIB-67 cell line were stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dashed line histogram or Alexa Fluor 647 Rat Anti-Mouse CD14 antibody Cat. No. 570368/570438; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD14 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD14 expression on viable Mouse thioglycolate-elicited peritoneal exudate cell populations. C57BL/6 Mouse peritoneal exudate cells were isolated 3 days post-stimulation by intraperitoneal injection of thioglycolate solution Thio-PEC and were preincubated with BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 .The cells were then stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD14 antibody Cat. No. 570368/570438; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD14 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD14 expression on viable J774A.1 cells. Cells from the J774A Mouse macrophage, ATCC TIB-67 cell line were stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dashed line histogram or Alexa Fluor 647 Rat Anti-Mouse CD14 antibody Cat. No. 570368/570438; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD14 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD14 expression on viable Mouse thioglycolate-elicited peritoneal exudate cell populations. C57BL/6 Mouse peritoneal exudate cells were isolated 3 days post-stimulation by intraperitoneal injection of thioglycolate solution Thio-PEC and were preincubated with BD Pharmingen Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141 .The cells were then stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD14 antibody Cat. No. 570368/570438; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. A bivariate pseudocolor density plot showing the correlated expression of CD14 or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable DAPI-negative Thio-PECs. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.