BD PharmingenTM Alexa FluorTM 647 Rat Anti-Mouse CD45

Description

Flow cytometric analysis of CD45 expression on mouse splenocytes. Splenic leucocytes from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were either unstained for a Fluorescence-Minus-One FMO control or stained with Alexa Fluor 647 Rat Anti-Mouse CD45 antibody Cat No. 567376; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or FMO control was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45 expression on mouse splenocytes. Splenic leucocytes from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were either unstained for a Fluorescence-Minus-One FMO control or stained with Alexa Fluor 647 Rat Anti-Mouse CD45 antibody Cat No. 567376; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or FMO control was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45 expression on mouse splenocytes. Splenic leucocytes from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were either unstained for a Fluorescence-Minus-One FMO control or stained with Alexa Fluor 647 Rat Anti-Mouse CD45 antibody Cat No. 567376; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD45 expression or FMO control was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.