BD PharmingenTM Alexa FluorTM 647 Rat Anti-Mouse CD98

Description

Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh Unstimulated; Left Panel or 48 hours after Concanavalin A Con A mitogenic stimulation Stimulated; Right Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dotted line histograms or Alexa Fluor 647 Rat Anti-Mouse CD98 antibody Cat No. 568193/568194; solid line histograms at 0.5 mug/test. The fluorescence histograms showing CD98 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh Unstimulated; Left Panel or 48 hours after Concanavalin A Con A mitogenic stimulation Stimulated; Right Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dotted line histograms or Alexa Fluor 647 Rat Anti-Mouse CD98 antibody Cat No. 568193/568194; solid line histograms at 0.5 mug/test. The fluorescence histograms showing CD98 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh Unstimulated; Left Panel or 48 hours after Concanavalin A Con A mitogenic stimulation Stimulated; Right Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dotted line histograms or Alexa Fluor 647 Rat Anti-Mouse CD98 antibody Cat No. 568193/568194; solid line histograms at 0.5 mug/test. The fluorescence histograms showing CD98 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh Unstimulated; Left Panel or 48 hours after Concanavalin A Con A mitogenic stimulation Stimulated; Right Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dotted line histograms or Alexa Fluor 647 Rat Anti-Mouse CD98 antibody Cat No. 568193/568194; solid line histograms at 0.5 mug/test. The fluorescence histograms showing CD98 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh Unstimulated; Left Panel or 48 hours after Concanavalin A Con A mitogenic stimulation Stimulated; Right Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dotted line histograms or Alexa Fluor 647 Rat Anti-Mouse CD98 antibody Cat No. 568193/568194; solid line histograms at 0.5 mug/test. The fluorescence histograms showing CD98 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD98 expression on unstimulated or stimulated Mouse splenic leucocytes. BALB/c mouse splenic leucocytes were tested fresh Unstimulated; Left Panel or 48 hours after Concanavalin A Con A mitogenic stimulation Stimulated; Right Panel . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dotted line histograms or Alexa Fluor 647 Rat Anti-Mouse CD98 antibody Cat No. 568193/568194; solid line histograms at 0.5 mug/test. The fluorescence histograms showing CD98 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable mouse leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.