BD PharmingenTM Alexa FluorTM 647 Rat Anti-Mouse iNOS

Description

Multiparameter flow cytometric analysis of iNOS expression in stimulated C57BL/6 Mouse thioglycolate-elicited peritoneal exudate cells. C57BL/6 thioglycollate-elicited peritoneal cells Thio-PECs were stimulated with Recombinant Mouse IFN-gamma protein Cat. No. 554587 and lipopolysaccharide LPS overnight 37C . Cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse F4/80 antibody Cat. No. 565614 and with either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 571480; Left Plot or Alexa Fluor 647 Rat Anti-Mouse iNOS antibody Cat. No. 572305/572306; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of iNOS or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of iNOS expression in stimulated C57BL/6 Mouse thioglycolate-elicited peritoneal exudate cells. C57BL/6 thioglycollate-elicited peritoneal cells Thio-PECs were stimulated with Recombinant Mouse IFN-gamma protein Cat. No. 554587 and lipopolysaccharide LPS overnight 37C . Cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse F4/80 antibody Cat. No. 565614 and with either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 571480; Left Plot or Alexa Fluor 647 Rat Anti-Mouse iNOS antibody Cat. No. 572305/572306; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of iNOS or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of iNOS expression in stimulated C57BL/6 Mouse thioglycolate-elicited peritoneal exudate cells. C57BL/6 thioglycollate-elicited peritoneal cells Thio-PECs were stimulated with Recombinant Mouse IFN-gamma protein Cat. No. 554587 and lipopolysaccharide LPS overnight 37C . Cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse F4/80 antibody Cat. No. 565614 and with either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 571480; Left Plot or Alexa Fluor 647 Rat Anti-Mouse iNOS antibody Cat. No. 572305/572306; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of iNOS or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of iNOS expression in stimulated C57BL/6 Mouse thioglycolate-elicited peritoneal exudate cells. C57BL/6 thioglycollate-elicited peritoneal cells Thio-PECs were stimulated with Recombinant Mouse IFN-gamma protein Cat. No. 554587 and lipopolysaccharide LPS overnight 37C . Cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse F4/80 antibody Cat. No. 565614 and with either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 571480; Left Plot or Alexa Fluor 647 Rat Anti-Mouse iNOS antibody Cat. No. 572305/572306; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of iNOS or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of iNOS expression in stimulated C57BL/6 Mouse thioglycolate-elicited peritoneal exudate cells. C57BL/6 thioglycollate-elicited peritoneal cells Thio-PECs were stimulated with Recombinant Mouse IFN-gamma protein Cat. No. 554587 and lipopolysaccharide LPS overnight 37C . Cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse F4/80 antibody Cat. No. 565614 and with either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 571480; Left Plot or Alexa Fluor 647 Rat Anti-Mouse iNOS antibody Cat. No. 572305/572306; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of iNOS or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of iNOS expression in stimulated C57BL/6 Mouse thioglycolate-elicited peritoneal exudate cells. C57BL/6 thioglycollate-elicited peritoneal cells Thio-PECs were stimulated with Recombinant Mouse IFN-gamma protein Cat. No. 554587 and lipopolysaccharide LPS overnight 37C . Cells were harvested, washed, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed, and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with BD Horizon BUV395 Rat Anti-Mouse F4/80 antibody Cat. No. 565614 and with either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 571480; Left Plot or Alexa Fluor 647 Rat Anti-Mouse iNOS antibody Cat. No. 572305/572306; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of iNOS or Ig Isotype control staining versus F4/80 was derived from gated events with the forward and side-light scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.