BD PharmingenTM Alexa FluorTM 700 Mouse Anti-Human CD45

Description

Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with either Alexa Fluor 700 Mouse IgG1, kappa Isotype Control Cat. No. 557882; Left Plot or Alexa Fluor 700 Mouse Anti-Human CD45 antibody Cat. No. 570762/570840; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with either Alexa Fluor 700 Mouse IgG1, kappa Isotype Control Cat. No. 557882; Left Plot or Alexa Fluor 700 Mouse Anti-Human CD45 antibody Cat. No. 570762/570840; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with either Alexa Fluor 700 Mouse IgG1, kappa Isotype Control Cat. No. 557882; Left Plot or Alexa Fluor 700 Mouse Anti-Human CD45 antibody Cat. No. 570762/570840; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with either Alexa Fluor 700 Mouse IgG1, kappa Isotype Control Cat. No. 557882; Left Plot or Alexa Fluor 700 Mouse Anti-Human CD45 antibody Cat. No. 570762/570840; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with either Alexa Fluor 700 Mouse IgG1, kappa Isotype Control Cat. No. 557882; Left Plot or Alexa Fluor 700 Mouse Anti-Human CD45 antibody Cat. No. 570762/570840; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with either Alexa Fluor 700 Mouse IgG1, kappa Isotype Control Cat. No. 557882; Left Plot or Alexa Fluor 700 Mouse Anti-Human CD45 antibody Cat. No. 570762/570840; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD45 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.