BD PharmingenTM Alexa FluorTM 700 Rat Anti-Mouse I-A/I-E

Description

Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either Alexa Fluor 700 Rat IgG2b, kappa Isotype Control Cat. No. 557964; Left Plot or Alexa Fluor 700 Rat Anti-Mouse I-A/I-E antibody Cat. No. 570802/570878; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either Alexa Fluor 700 Rat IgG2b, kappa Isotype Control Cat. No. 557964; Left Plot or Alexa Fluor 700 Rat Anti-Mouse I-A/I-E antibody Cat. No. 570802/570878; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either Alexa Fluor 700 Rat IgG2b, kappa Isotype Control Cat. No. 557964; Left Plot or Alexa Fluor 700 Rat Anti-Mouse I-A/I-E antibody Cat. No. 570802/570878; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either Alexa Fluor 700 Rat IgG2b, kappa Isotype Control Cat. No. 557964; Left Plot or Alexa Fluor 700 Rat Anti-Mouse I-A/I-E antibody Cat. No. 570802/570878; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either Alexa Fluor 700 Rat IgG2b, kappa Isotype Control Cat. No. 557964; Left Plot or Alexa Fluor 700 Rat Anti-Mouse I-A/I-E antibody Cat. No. 570802/570878; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553064 and with either Alexa Fluor 700 Rat IgG2b, kappa Isotype Control Cat. No. 557964; Left Plot or Alexa Fluor 700 Rat Anti-Mouse I-A/I-E antibody Cat. No. 570802/570878; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.