BD PharmingenTM APC-CyTM7 Rat Anti-Mouse CD45R

Description

Multicolor flow cytometric analysis of CD45R expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and with either APC-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552770; Left Plot or APC-Cy7 Rat Anti-Mouse CD45R antibody Cat. No. 552094; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD45R or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and with either APC-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552770; Left Plot or APC-Cy7 Rat Anti-Mouse CD45R antibody Cat. No. 552094; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD45R or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and with either APC-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552770; Left Plot or APC-Cy7 Rat Anti-Mouse CD45R antibody Cat. No. 552094; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD45R or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD45R expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and with either APC-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552770; Left Plot or APC-Cy7 Rat Anti-Mouse CD45R antibody Cat. No. 552094; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD45R or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD45R expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and with either APC-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552770; Left Plot or APC-Cy7 Rat Anti-Mouse CD45R antibody Cat. No. 552094; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD45R or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553062/553061/561827 and with either APC-Cy7 Rat IgG2a, kappa Isotype Control Cat. No. 552770; Left Plot or APC-Cy7 Rat Anti-Mouse CD45R antibody Cat. No. 552094; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD45R or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry and data analysis were performed using a BD FACSCanto II System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.