BD PharmingenTM APC-CyTM7 Rat Anti-Mouse IL-17A

Description

Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with APC-Cy7 Rat Anti-Mouse IL-17A Cat. No. 560821; middle and right panels or APC-Cy7 Rat IgG1, kappa Isotype Control Cat. No. 560534; left panel using the BD Cytofix/Cytoperm Method for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Bivariate flow cytometric dot plots showing correlated expression patterns of IL-17A APC-Cy7 versus cellular autofluorescence FL1 were derived from gated events with the forward and side light-scatter characteristics of viable cells. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control FL1 and the APC-Cy7 Rat IgG1, kappa Isotype Control staining. Flow cytometry was performed on a BD LSR II Flow Cytometer System. Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with APC-Cy7 Rat Anti-Mouse IL-17A Cat. No. 560821; middle and right panels or APC-Cy7 Rat IgG1, kappa Isotype Control Cat. No. 560534; left panel using the BD Cytofix/Cytoperm Method for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Bivariate flow cytometric dot plots showing correlated expression patterns of IL-17A APC-Cy7 versus cellular autofluorescence FL1 were derived from gated events with the forward and side light-scatter characteristics of viable cells. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control FL1 and the APC-Cy7 Rat IgG1, kappa Isotype Control staining. Flow cytometry was performed on a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IL-17A-producing cells within a stimulated mouse EL4 thymoma cell population. EL4 cells were stimulated left and middle panels or unstimulated right panel with PMA 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 for 5 hours. The cells were fixed, permeabilized and subsequently stained with APC-Cy7 Rat Anti-Mouse IL-17A Cat. No. 560821; middle and right panels or APC-Cy7 Rat IgG1, kappa Isotype Control Cat. No. 560534; left panel using the BD Cytofix/Cytoperm Method for Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis. Bivariate flow cytometric dot plots showing correlated expression patterns of IL-17A APC-Cy7 versus cellular autofluorescence FL1 were derived from gated events with the forward and side light-scatter characteristics of viable cells. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control FL1 and the APC-Cy7 Rat IgG1, kappa Isotype Control staining. Flow cytometry was performed on a BD LSR II Flow Cytometer System.