BD PharmingenTM APC-CyTM7 Rat Anti-Mouse Ly-6A/E

Description

Flow cytometric analysis of Ly-6A/E on stimulated mouse splenocytes. BALB/c splenocytes were stimulated with 2.5-5.0 mug/mL concanavilin A Sigma-Aldrich cat. no. C2010 for 48 hours and were subsequently stained either with a APC-Cy7 Rat IgG2a, kappa isotype control Cat. No. 552770, unshaded or with the APC-Cy7 Rat Anti-Mouse Ly-6A/E antibody Cat. No. 560654, shaded . Histograms were derived from gated events based on light scattering characteristics for splenocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis of Ly-6A/E on stimulated mouse splenocytes. BALB/c splenocytes were stimulated with 2.5-5.0 mug/mL concanavilin A Sigma-Aldrich cat. no. C2010 for 48 hours and were subsequently stained either with a APC-Cy7 Rat IgG2a, kappa isotype control Cat. No. 552770, unshaded or with the APC-Cy7 Rat Anti-Mouse Ly-6A/E antibody Cat. No. 560654, shaded . Histograms were derived from gated events based on light scattering characteristics for splenocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis of Ly-6A/E on stimulated mouse splenocytes. BALB/c splenocytes were stimulated with 2.5-5.0 mug/mL concanavilin A Sigma-Aldrich cat. no. C2010 for 48 hours and were subsequently stained either with a APC-Cy7 Rat IgG2a, kappa isotype control Cat. No. 552770, unshaded or with the APC-Cy7 Rat Anti-Mouse Ly-6A/E antibody Cat. No. 560654, shaded . Histograms were derived from gated events based on light scattering characteristics for splenocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.