BD PharmingenTM APC-H7 Mouse Anti-Human IgG

Description

Flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight in order to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with PE Mouse anti-Human CD19 Cat. No. 555413 and either APC-H7 Mouse anti-Human IgG antibody Cat. No. 561297; Right Panel or with an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; Left Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight in order to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with PE Mouse anti-Human CD19 Cat. No. 555413 and either APC-H7 Mouse anti-Human IgG antibody Cat. No. 561297; Right Panel or with an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; Left Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight in order to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with PE Mouse anti-Human CD19 Cat. No. 555413 and either APC-H7 Mouse anti-Human IgG antibody Cat. No. 561297; Right Panel or with an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; Left Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight in order to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with PE Mouse anti-Human CD19 Cat. No. 555413 and either APC-H7 Mouse anti-Human IgG antibody Cat. No. 561297; Right Panel or with an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; Left Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight in order to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with PE Mouse anti-Human CD19 Cat. No. 555413 and either APC-H7 Mouse anti-Human IgG antibody Cat. No. 561297; Right Panel or with an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; Left Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were washed and cultured in complete tissue culture medium overnight in order to minimize nonspecific immunofluorescent staining. The cells were harvested and stained with PE Mouse anti-Human CD19 Cat. No. 555413 and either APC-H7 Mouse anti-Human IgG antibody Cat. No. 561297; Right Panel or with an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; Left Panel . Two-color flow cytometric dot plots showing the correlated expression of cell surface IgG or Ig isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.