BD PharmingenTM APC-H7 Mouse Anti-Human MIP-1beta

Description

Flow cytometric analysis of MIP-1beta expressed in human peripheral blood mononuclear cells PBMC . Human PBMC were either unstimulated Left Panel or stimulated Right Panel with 20 ng/mL Recombinant Human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The PBMC were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; dashed line histogram or with the APC-H7 Mouse Anti-Human MIP-1beta antibody Cat. No. 561280; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of MIP-1beta expressed in human peripheral blood mononuclear cells PBMC . Human PBMC were either unstimulated Left Panel or stimulated Right Panel with 20 ng/mL Recombinant Human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The PBMC were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; dashed line histogram or with the APC-H7 Mouse Anti-Human MIP-1beta antibody Cat. No. 561280; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of MIP-1beta expressed in human peripheral blood mononuclear cells PBMC . Human PBMC were either unstimulated Left Panel or stimulated Right Panel with 20 ng/mL Recombinant Human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The PBMC were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; dashed line histogram or with the APC-H7 Mouse Anti-Human MIP-1beta antibody Cat. No. 561280; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of MIP-1beta expressed in human peripheral blood mononuclear cells PBMC . Human PBMC were either unstimulated Left Panel or stimulated Right Panel with 20 ng/mL Recombinant Human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The PBMC were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; dashed line histogram or with the APC-H7 Mouse Anti-Human MIP-1beta antibody Cat. No. 561280; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of MIP-1beta expressed in human peripheral blood mononuclear cells PBMC . Human PBMC were either unstimulated Left Panel or stimulated Right Panel with 20 ng/mL Recombinant Human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The PBMC were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; dashed line histogram or with the APC-H7 Mouse Anti-Human MIP-1beta antibody Cat. No. 561280; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of MIP-1beta expressed in human peripheral blood mononuclear cells PBMC . Human PBMC were either unstimulated Left Panel or stimulated Right Panel with 20 ng/mL Recombinant Human IFN-gamma Cat. No. 554616 for one hour followed by overnight incubation with 1 mug/mL LPS Sigma-Aldrich, Cat. No. L-8272 in the presence of 2 muM BD GolgiStop Protein Transport Inhibitor Cat. No. 554724 . The PBMC were harvested, fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either an APC-H7 Mouse IgG1, kappa Isotype Control Cat. No. 560167; dashed line histogram or with the APC-H7 Mouse Anti-Human MIP-1beta antibody Cat. No. 561280; solid line histogram . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.