BD PharmingenTM APC Hamster Anti-Mouse CD137 (4-1BB)

Description

Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leukocytes. Concanavalin A Con A -activated 3 days BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD25 antibody Cat. No. 553075 and with either no APC conjugated antibody Autofluorescence control; Left Plot or APC Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 570316/570394; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or cellular Autofluorescence versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leukocytes. Concanavalin A Con A -activated 3 days BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD25 antibody Cat. No. 553075 and with either no APC conjugated antibody Autofluorescence control; Left Plot or APC Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 570316/570394; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or cellular Autofluorescence versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leukocytes. Concanavalin A Con A -activated 3 days BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD25 antibody Cat. No. 553075 and with either no APC conjugated antibody Autofluorescence control; Left Plot or APC Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 570316/570394; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or cellular Autofluorescence versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leukocytes. Concanavalin A Con A -activated 3 days BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD25 antibody Cat. No. 553075 and with either no APC conjugated antibody Autofluorescence control; Left Plot or APC Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 570316/570394; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or cellular Autofluorescence versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leukocytes. Concanavalin A Con A -activated 3 days BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD25 antibody Cat. No. 553075 and with either no APC conjugated antibody Autofluorescence control; Left Plot or APC Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 570316/570394; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or cellular Autofluorescence versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD137 4-1BB expression on viable stimulated Mouse splenic leukocytes. Concanavalin A Con A -activated 3 days BALB/c Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The leukocytes were then stained with PE Rat Anti-Mouse CD25 antibody Cat. No. 553075 and with either no APC conjugated antibody Autofluorescence control; Left Plot or APC Hamster Anti-Mouse CD137 4-1BB antibody Cat. No. 570316/570394; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD137 4-1BB or cellular Autofluorescence versus CD25 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.