BD PharmingenTM APC Hamster Anti-Mouse CD152

Description

Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A ConA . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either APC Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Panel or APC Hamster Anti-Mouse CD152 antibody Cat. No. 564331; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A ConA . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either APC Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Panel or APC Hamster Anti-Mouse CD152 antibody Cat. No. 564331; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A ConA . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either APC Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Panel or APC Hamster Anti-Mouse CD152 antibody Cat. No. 564331; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A ConA . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either APC Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Panel or APC Hamster Anti-Mouse CD152 antibody Cat. No. 564331; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A ConA . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either APC Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Panel or APC Hamster Anti-Mouse CD152 antibody Cat. No. 564331; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD152 expression on activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 72 hours with concanavalin A ConA . The cells were harvested and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either APC Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Panel or APC Hamster Anti-Mouse CD152 antibody Cat. No. 564331; Right Panel . Two-color flow cytometric dot plots showing the correlated expression of CD152 or Ig Isotype control staining versus CD8a were derived from gated events with the forward and side light-scatter characteristics of viable activated leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.