BD PharmingenTM APC Hamster Anti-Mouse CD3e

Description

Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553030/553031/561966 antibodies and either APC Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Plot or APC Hamster Anti-Mouse CD3e antibody Cat. No. 561826/553066; Right Plot at 1.0 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553030/553031/561966 antibodies and either APC Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Plot or APC Hamster Anti-Mouse CD3e antibody Cat. No. 561826/553066; Right Plot at 1.0 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553030/553031/561966 antibodies and either APC Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Plot or APC Hamster Anti-Mouse CD3e antibody Cat. No. 561826/553066; Right Plot at 1.0 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553030/553031/561966 antibodies and either APC Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Plot or APC Hamster Anti-Mouse CD3e antibody Cat. No. 561826/553066; Right Plot at 1.0 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553030/553031/561966 antibodies and either APC Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Plot or APC Hamster Anti-Mouse CD3e antibody Cat. No. 561826/553066; Right Plot at 1.0 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on mouse splenocytes. Mouse splenic leucocytes were stained with FITC Rat Anti-Mouse CD4 Cat. No. 553046/553047/561835 and FITC Rat Anti-Mouse CD8a Cat. No. 553030/553031/561966 antibodies and either APC Armenian Hamster IgG1, kappa Isotype Control Cat. No. 553974; Left Plot or APC Hamster Anti-Mouse CD3e antibody Cat. No. 561826/553066; Right Plot at 1.0 mug/test. Bivariate pseudocolor density plots showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a were derived from gated events with the forward and side light-scatter characteristics of intact splenic leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.