BD PharmingenTM APC Mouse Anti-Human CCR7 (CD197)

Description

Flow cytometric analysis of CCR7 CD197 on human peripheral blood leukocytes and lymphocytes. Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 564724 , BD Horizon BV421 Mouse Anti-Human CD8 clone RPA-T8, Cat. No. 562429 or 562428 , FITC Mouse Anti-Human CD45RA clone HI100, Cat. No. 561882 or 555488 , and either APC Mouse IgG1, kappa Isotype Control Cat. No.340442 or APC Mouse Anti-Human CCR7 CD197 Cat. No. 566762 or 566763 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Plots 1 and 2: The contour plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The contour plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The contour plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis of CCR7 CD197 on human peripheral blood leukocytes and lymphocytes. Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 564724 , BD Horizon BV421 Mouse Anti-Human CD8 clone RPA-T8, Cat. No. 562429 or 562428 , FITC Mouse Anti-Human CD45RA clone HI100, Cat. No. 561882 or 555488 , and either APC Mouse IgG1, kappa Isotype Control Cat. No.340442 or APC Mouse Anti-Human CCR7 CD197 Cat. No. 566762 or 566763 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Plots 1 and 2: The contour plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The contour plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The contour plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Flow cytometric analysis of CCR7 CD197 on human peripheral blood leukocytes and lymphocytes. Whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 564724 , BD Horizon BV421 Mouse Anti-Human CD8 clone RPA-T8, Cat. No. 562429 or 562428 , FITC Mouse Anti-Human CD45RA clone HI100, Cat. No. 561882 or 555488 , and either APC Mouse IgG1, kappa Isotype Control Cat. No.340442 or APC Mouse Anti-Human CCR7 CD197 Cat. No. 566762 or 566763 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACS LSRFortessa X20 Flow Cytometer and FlowJo software. Plots 1 and 2: The contour plots showing Ig isotype control or CCR7 CD197 staining versus side light-scatter were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Plot 3: The contour plot showing CCR7 CD197 staining versus CD45RA was derived from CD4-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes. Please note that we have observed considerable donor-to-donor variation in the proportion of CCR7 CD197 -negative CD4-positive lymphocytes. Plot 4: The contour plot showing CCR7 CD197 staining versus CD45RA was derived from CD8-positive T cell gated events with the forward and side light-scatter characteristics of viable lymphocytes.