BD PharmingenTM APC Mouse Anti-Human CD133

Description

CD133 expression on human WERI-Rb-1cells Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; dashed line histogram or APC Mouse Anti-Human CD133 antibody Cat. No. 566596/566597; solid line histogram at 0.06 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with PE Mouse Anti-Human CD34 antibody Cat. No. 348057 and either APC Mouse IgG1, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 Right Plot at 0.06 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and either APC Mouse IgG1, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.06 mug/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 or Ig Isotype control staining were similarly derived for viable PBL. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. CD133 expression on human WERI-Rb-1cells Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; dashed line histogram or APC Mouse Anti-Human CD133 antibody Cat. No. 566596/566597; solid line histogram at 0.06 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with PE Mouse Anti-Human CD34 antibody Cat. No. 348057 and either APC Mouse IgG1, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 Right Plot at 0.06 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and either APC Mouse IgG1, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.06 mug/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 or Ig Isotype control staining were similarly derived for viable PBL. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More CD133 expression on human WERI-Rb-1cells Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; dashed line histogram or APC Mouse Anti-Human CD133 antibody Cat. No. 566596/566597; solid line histogram at 0.06 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with forward and side light-scatter characteristics of viable cells. CD133 expression on human peripheral blood mononuclear cells Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with PE Mouse Anti-Human CD34 antibody Cat. No. 348057 and either APC Mouse IgG1, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 Right Plot at 0.06 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells before analysis. Pseudocolor dot plots showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and either APC Mouse IgG1, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.06 mug/test followed by Cell Viability 7-AAD Solution addition before analysis. Pseudocolor dot plots showing the correlated expression of CD45 versus CD133 or Ig Isotype control staining were similarly derived for viable PBL. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.