BD PharmingenTM APC Mouse Anti-Human CD133

Description

CD133 expression on retinoblastoma cell line and Human peripheral blood mononuclear cells PBMC . Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either APC Mouse IgG2b, kappa Isotype Control Cat. No. 565381; dashed line histogram or APC Mouse Anti-Human CD133 antibody Cat. No. 567909/567910 solid line histogram at 0.25 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon CD34 BV421 antibody Cat. No. 663981 and with either APC Mouse IgG2b, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and with either APC Mouse IgG2b, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.25 mug/test followed by BD Via-Probe Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD45 was similarly derived for viable 7-AAD-negative PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. CD133 expression on retinoblastoma cell line and Human peripheral blood mononuclear cells PBMC . Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either APC Mouse IgG2b, kappa Isotype Control Cat. No. 565381; dashed line histogram or APC Mouse Anti-Human CD133 antibody Cat. No. 567909/567910 solid line histogram at 0.25 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon CD34 BV421 antibody Cat. No. 663981 and with either APC Mouse IgG2b, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and with either APC Mouse IgG2b, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.25 mug/test followed by BD Via-Probe Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD45 was similarly derived for viable 7-AAD-negative PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More CD133 expression on retinoblastoma cell line and Human peripheral blood mononuclear cells PBMC . Panel 1. Cells from the WERI-Rb-1 Retinoblastoma, ATCC HTB-169 cell line were stained with either APC Mouse IgG2b, kappa Isotype Control Cat. No. 565381; dashed line histogram or APC Mouse Anti-Human CD133 antibody Cat. No. 567909/567910 solid line histogram at 0.25 mug/test. The fluorescence histogram showing CD133 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable cells. Panel 2A. Human peripheral blood mononuclear cells PBMC were stained with BD Horizon CD34 BV421 antibody Cat. No. 663981 and with either APC Mouse IgG2b, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD34 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative peripheral blood lymphocytes PBL . Panel 2B. Human PBMC were similarly stained with BD Horizon BUV395 Mouse Anti-Human CD45 antibody Cat. No. 563791/563792 and with either APC Mouse IgG2b, kappa Isotype Control Left Plot or APC Mouse Anti-Human CD133 antibody Right Plot at 0.25 mug/test followed by BD Via-Probe Cell Viability 7-AAD Solution addition before analysis. The pseudocolor density plot showing the correlated expression of CD133 or Ig Isotype control staining versus CD45 was similarly derived for viable 7-AAD-negative PBL. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.