BD PharmingenTM APC Mouse Anti-Human CD69

Description

Flow cytometric analysis for CD69 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . PBMC from Rhesus macaque were stimulated for 4-6 hours with 20 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a APC Mouse IgG1, kappa isotype control Cat. No. 550854; shaded histogram or with the APC Mouse Anti-Human CD69 antibody Cat. No. 560711/555533/560967; unshaded histogram . Histograms were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for CD69 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . PBMC from Rhesus macaque were stimulated for 4-6 hours with 20 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a APC Mouse IgG1, kappa isotype control Cat. No. 550854; shaded histogram or with the APC Mouse Anti-Human CD69 antibody Cat. No. 560711/555533/560967; unshaded histogram . Histograms were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis for CD69 in stimulated Rhesus macaque peripheral blood mononuclear cells PBMC . PBMC from Rhesus macaque were stimulated for 4-6 hours with 20 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a APC Mouse IgG1, kappa isotype control Cat. No. 550854; shaded histogram or with the APC Mouse Anti-Human CD69 antibody Cat. No. 560711/555533/560967; unshaded histogram . Histograms were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.