BD PharmingenTM APC Mouse Anti-Human CD86 (B7-2)

Description

Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations and Daudi cells. Left and Center Plots - Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571636/571715; Center Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either APC Mouse IgG1, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human CD86 B7-2 antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations and Daudi cells. Left and Center Plots - Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571636/571715; Center Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either APC Mouse IgG1, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human CD86 B7-2 antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations and Daudi cells. Left and Center Plots - Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571636/571715; Center Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either APC Mouse IgG1, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human CD86 B7-2 antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations and Daudi cells. Left and Center Plots - Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571636/571715; Center Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either APC Mouse IgG1, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human CD86 B7-2 antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations and Daudi cells. Left and Center Plots - Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571636/571715; Center Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either APC Mouse IgG1, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human CD86 B7-2 antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations and Daudi cells. Left and Center Plots - Human whole blood was first treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 . The leukocytes were then stained with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571636/571715; Center Plot . The bivariate pseudocolor density plot showing the correlated expression of CD86 B7-2 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Right Plot - Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CCL-213 cell line were stained with either APC Mouse IgG1, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human CD86 B7-2 antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software.