BD PharmingenTM APC Mouse Anti-Human DNAM-1 (CD226)

Description

Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567857/567858; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 was derived from gated events based on the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567857/567858; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 was derived from gated events based on the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567857/567858; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 was derived from gated events based on the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567857/567858; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 was derived from gated events based on the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567857/567858; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 was derived from gated events based on the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Two-color flow cytometric analysis of DNAM-1 CD226 expression on human peripheral blood leucocytes. Platelet-depleted human whole blood was stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333 and with either APC Mouse IgG1, kappa Isotype Control Cat. No. 554681; Left Plot or APC Mouse Anti-Human DNAM-1 CD226 antibody Cat. No. 567857/567858; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing the correlated expression of DNAM-1 CD226 or Ig Isotype control staining versus CD3 was derived from gated events based on the forward and side-light scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.