BD PharmingenTM APC Mouse Anti-Human HLA-A3

Description

Multiparameter flow cytometric analysis of HLA-A3 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-A3 antibody Cat. No. 570460/570462; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A3 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of HLA-A3 expression on viable Jurkat cells. Jurkat cells were stained with APC Mouse IgG2a, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human HLA-A3 antibody solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing HLA-A3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of HLA-A3 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-A3 antibody Cat. No. 570460/570462; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A3 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of HLA-A3 expression on viable Jurkat cells. Jurkat cells were stained with APC Mouse IgG2a, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human HLA-A3 antibody solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing HLA-A3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of HLA-A3 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-A3 antibody Cat. No. 570460/570462; Right Plot at 0.5 mug/test. Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of HLA-A3 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of HLA-A3 expression on viable Jurkat cells. Jurkat cells were stained with APC Mouse IgG2a, kappa Isotype Control dashed line histogram or APC Mouse Anti-Human HLA-A3 antibody solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing HLA-A3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.