BD PharmingenTM APC Mouse Anti-Human HLA-ABC

Description

Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-ABC antibody Cat. No. 570825/570901; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-ABC antibody Cat. No. 570825/570901; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-ABC antibody Cat. No. 570825/570901; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-ABC antibody Cat. No. 570825/570901; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-ABC antibody Cat. No. 570825/570901; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of HLA-ABC expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either APC Mouse IgG2a, kappa Isotype Control Cat. No. 550882; Left Plot or APC Mouse Anti-Human HLA-ABC antibody Cat. No. 570825/570901; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of HLA-ABC or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessaX-20 Cell Analyzer System and FlowJo Software.