BD PharmingenTM APC Mouse Anti-Human IL-3Ralpha (CD123)

Description

Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with FITC Mouse Anti-Human CD45RO Cat No. 555492/561887; Bottom Plots and with either APC Mouse IgG1, kappa Isotype Control Cat No. 550854/555751; Left Plots or APC Mouse Anti-Human IL-3Ralpha CD123 antibody Cat No. 567275/567276; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with FITC Mouse Anti-Human CD45RO Cat No. 555492/561887; Bottom Plots and with either APC Mouse IgG1, kappa Isotype Control Cat No. 550854/555751; Left Plots or APC Mouse Anti-Human IL-3Ralpha CD123 antibody Cat No. 567275/567276; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Human whole blood was stained with FITC Mouse Anti-Human CD45RO Cat No. 555492/561887; Bottom Plots and with either APC Mouse IgG1, kappa Isotype Control Cat No. 550854/555751; Left Plots or APC Mouse Anti-Human IL-3Ralpha CD123 antibody Cat No. 567275/567276; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Upper Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes. Lower Plots: Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus CD45RO were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.